https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47613 Tue 24 Jan 2023 11:43:18 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36571 Tue 09 Jun 2020 11:40:42 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39495 Crocodylus porosus as a tractable model for understanding crocodilian sperm physiology. In extending our systematic characterisation of saltwater crocodile spermatozoa, in this study we examined the development of motility during sperm transport through the excurrent duct system of the male crocodile. The results show that approximately 20% of crocodile testicular spermatozoa are immediately motile but experience a gradient of increasing motility (percentage motile and rate of movement) as they transit the male reproductive tract (epididymis). Moreover, we confirmed that, as in ejaculated crocodile spermatozoa, increased intracellular cAMP levels promoted a significant and sustained enhancement of sperm motility regardless of whether the cells were isolated from the testis or epididymis. Along with the development of artificial reproductive technologies, this research paves the way for the opportunistic recovery, storage and potential utilisation of post-mortem spermatozoa from genetically valuable animals.]]> Tue 09 Aug 2022 14:34:34 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:40953 Tue 07 Nov 2023 09:51:49 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:43870 Tue 04 Oct 2022 12:28:50 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:45372 Chd4 expression significantly impairs SSC regenerative capacity, causing a ∼50% reduction in colonization of recipient testes. An scRNA-seq comparison revealed reduced expression of “self-renewal” genes following Chd4 knockdown, along with increased expression of signature progenitor genes. Co-immunoprecipitation analyses demonstrated that CHD4 regulates gene expression in spermatogonia not only through its traditional association with the remodeling complex NuRD, but also via interaction with the GDNF-responsive transcription factor SALL4. Cumulatively, the results of this study depict a previously unappreciated role for CHD4 in controlling fate decisions in the spermatogonial pool.]]> Thu 27 Oct 2022 12:14:10 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51259 Mon 28 Aug 2023 13:50:03 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:36974 Fri 24 Jul 2020 14:55:41 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49362 Fri 12 May 2023 12:35:13 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:38175 Fri 06 Aug 2021 14:14:50 AEST ]]>